The CCK8 assay showed that pcDNA3.1-PELATON increased U251 cell proliferation, comparable with that of pcDNA3.1 U251 cells, which have relatively low expression PELATON. Knockdown of PELATON inhibited the proliferation of PG-3 primary GBM cells, which have relatively high PELATON expression (Figure 5D). The gene discussed is PELATON; the disease is glioblastoma.