Recent studies have shown that EVs rich in PFKFB3, MMP-13, intercellular cell adhesion molecule-1 or thrombospondin-1 can enhance the release of VEGF-A, IL-8 and then downregulate junction-related proteins, which promote tumor angiogenesis and vascular permeability and become a potential channel system for distant metastasis of tumor cells (89–91). The gene discussed is PFKFB3; the disease is neoplasm.