Further experiments by Guiqin Hou and colleagues highlighted that shRNA inhibition of Rictor could reduce phosphorylation of AKTSer473 and Thr308 sites, and counteract activation of AKT (Ser473)/PRAS40 (Thr246) induced by LY294002, which significantly improved the sensitivity of ESCC cells to LY294002 in vitro and in vivo (86). The gene discussed is AKT1; the disease is esophageal squamous cell carcinoma.