Through the establishment of ECM1-overexpressing tumor xenograft mouse models, we further confirmed that ECM1 overexpression in HCT116 cells in vivo was associated with consistent increases in Vimentin expression together with E-cadherin downregulation, suggesting that ECM1 can regulate the EMT process and thereby promote CRC cell metastatic progression in vitro and in vivo. Here, VIM is linked to neoplasm.