To analyze the link between ECM1 and this process, we conducted Western blotting assays examining EMT marker protein expression in CRC cells, revealing ECM1 knockdown to enhance E-cadherin expression while suppressing Vimentin expression (Figures 5A, –C), whereas the opposite changes were observed upon ECM1 overexpression (Figures 5D, –F). This evidence concerns the gene VIM and colorectal carcinoma.