We then performed a Mito Stress test on freshly dissociated zebrafish tumor brains using the Seahorse XFp apparatus and acute injection of lactate (20 mM), UK5099 [an inhibitor of the mitochondrial pyruvate carrier (MPC), 2 μM], or AZ3695 (10 μM, to inhibit MCT1). Here, SLC16A1 is linked to neoplasm.