Having established the performance of our AT- and RF-based segmentation methods in silico and in a string phantom, next we sought to determine the influence of the chosen method in quantifying tumour vascular networks from size-matched breast cancer patient-derived xenograft (PDX) tumours of two subtypes (ER- n = 6; ER+ n = 8, total n = 14). Here, ESR1 is linked to breast carcinoma.