Our findings shed light on TCIRG1 nsSNPs, their conservation, impact on protein stability and functions, protein 3D structure, PTM potential sites, ligand binding sites, and gene-gene interactions with other genes, all of which could be useful in future TCIRG1 research to better understand its role in diseases such as osteopetrosis and congenital neutropenia. This evidence concerns the gene TCIRG1 and osteopetrosis.