Considering that female APOE-e4 carriers are more likely to progress from MCI to AD, develop AD more frequently than age-matched males, and have more brain atrophy and memory loss (Fleisher et al., 2008; Altmann et al., 2014; Sampedro et al., 2015), we next sought to determine if these biological factors impact CSF EV miRNA cargo. The gene discussed is APOE; the disease is Brain atrophy.