To the best of our knowledge, aside from the study herein, eight studies have investigated miRNA expression in CSF EVs from AD participants (Liu et al., 2014; Gui et al., 2015; Riancho et al., 2017; McKeever et al., 2018; Schneider et al., 2018; Jain et al., 2019; Kumar and Reddy, 2021; Tan et al., 2021), and we are the first to investigate the effects of sex and APOE-e4 status on CSF EVs and their miRNA cargo. This evidence concerns the gene APOE and Alzheimer disease.