Therefore, in this study, we employ middle-aged, brain-ABCA1 deficient (ABCA1−B/−B) and ABCA1-floxed (ABCA1fl/fl) control mice that were induced with T2DM and subjected to stroke, to test whether L-4F treatment initiated at 24 h after onset of ischemic stroke enhances neurological recovery. This evidence concerns the gene ABCA1 and type 2 diabetes mellitus.