Moreover, the results of western blotting experiments on liver tissues of fibrotic mice induced by miRNA agomir showed that α-SMA, Col 1 (α1), and Col 3 (α1) were upregulated at the protein levels (Figure 3C), indicating that liver fibrosis was promoted in mice injected with novel miRNA-33 agomir. The gene discussed is ACTA1; the disease is Hepatic fibrosis.