In order to verify whether the novel miRNA-33 agomir could promote mouse liver fibrosis as expected, we performed qPCR experiments on liver tissues of fibrotic mice induced by miRNA agomir, and the results showed that α-SMA, Col 1 (α1), and Col 3 (α1) were upregulated at mRNA level (Figure 3B). The gene discussed is ACTA1; the disease is Hepatic fibrosis.