To further validate the PD model eraIs1, we tested whether known neurodegenerative triggers, such as loss-of-function of Parkin and PINK1 and gain of function of human LRRK2, which are associated with familial forms of PD, would induce neuronal defects upon αSyn expression (Yao et al., 2010; Blauwendraat et al., 2020). Here, PRKN is linked to Parkinson disease.