According to the gray value calculation, the downregulation of TGF-β1, TGF-β2, Smad4, and p-Smad2 protein levels in the siRNA1 and siRNA2 groups was statistically significant compared to controls in U87 glioma cells (p < 0.05, Figure 6(b)). The gene discussed is TGFB2; the disease is glioma.