Herein, the activation of the TGF-β/Smad pathway and subsequent phosphorylation of Smad proteins were impaired by HOXC6 silencing, suggesting that HOXC6 exerts its regulatory effect on the migrative and invasive capacity of glioma cells by promoting the activation of the TGF-β/Smad pathway. The gene discussed is HOXC6; the disease is glioma.