The gene WIPI1 was excluded by applying Random Forest to continue the screening (Figure 4A), then ten key genes (STEAP3, HSPB1, MAP1LC3A, SOCS1, LOX, CAPG, CP, GDF15, CDKN1A, and CD44) were screened and the heat map of expression was plotted in GBM versus normal brain tissue groups. The gene discussed is GDF15; the disease is glioblastoma.