OM significantly upregulated ALP and RUNX2 expression (*p < 0.05) compared to the control group, whereas ALP and RUNX2 were significantly downregulated in VICs treated with different concentrations of AT-1 in OM medium (p < 0.05) (Figure 2A).We detected the protein levels of RUNX2 and ALP in VICs stimulated in OM for 48 h by Western blots. Here, RUNX2 is linked to ocular melanoma.