Table 3 exhibits the top 10 up-regulated signalling pathways associated with CHSY3 according to the GSEA score in STAD. We found that the transforming growth factor-beta (TGF-β), Wnt and Hedgehog signalling pathways were associated with the tumour mesenchymal pathway. The chemokine, Toll-like receptor and NOD-like receptor signalling pathways are involved in immune and inflammatory activation (Figure 4). Interestingly, high expression of CHSY3 in STAD did not perform a better prognosis. Here, TGFB1 is linked to gastric adenocarcinoma.