Based on the RT-qPCR results, AC109439.1 expression was low, and AGAP2-AS1, HOXC-AS1, and HOTAIR expression was high in glioma samples, while obvious differences in the expression levels of AL133415.1, AL035446.1, and CDK6-AS1 were not observed between glioma and normal tissues (Figure 10A). This evidence concerns the gene HOTAIR and glioma.