Our results revealed that the downregulated LINC01128 could decrease PTEN via sponging miR-25-3p, and then the signal response caused by the downregulation of PTEN would activate the PI3K/Akt pathway, which may lead to the development of EGFR-TKI resistance in NSCLC. The gene discussed is PTEN; the disease is non-small cell lung carcinoma.