In order to deeply explore the relationship between NRP1 and radiosensitivity in ESCC, GO (Gene Ontology) analysis (Supplementary Figure S4C) and gene set enrichment analysis (Supplementary Figure S4D) were performed and found that high NRP1 expression was closely and positively related to EMT activation, indicating EMT might account for the role of NRP1 in ESCC radiotherapy resistance. The gene discussed is NRP1; the disease is esophageal squamous cell carcinoma.