The goal of this study is to substantiate the clinicopathological significances for CXCL13 and M2 patterns (CD163+) in astrocytoma and to compare the potential utility of CXCL13 and CD163 as diagnostic biomarkers, alone and in combination, which discriminates between the different grades of astrocytoma (grades II, III, IV). This evidence concerns the gene CD163 and astrocytoma (excluding glioblastoma).