Overall, the expression of ANLN was found to be associated with the infiltration levels of CD8+ T cells in 13 tumor types, CD4+ T cells in 20 tumor types, B cells in 20 tumor types, macrophages in 20 tumor types, neutrophils in 26 tumor types, and NK cells in 15 tumor types (Fig. 6A–F, Additional file 4: Figure S4, Additional file 5: Figure S5, Additional file 6: Figure S6). This evidence concerns the gene ANLN and neoplasm.