Among 181 mutants, R153Q/L mutation was detected in five cases (uterine serous carcinoma, LUAD, cutaneous melanoma, mucinous adenocarcinoma of the colon and rectum, and HNSC) and induced a frame-shift mutation in ANLN, resulting in truncation of the protein (Fig. 4C). The gene discussed is ANLN; the disease is endometrial serous adenocarcinoma.