We used 35 U/ml as a cutoff based on recommendations of National Institute for Health and Care Excellence (NICE); this cutoff was derived from a prior study in which only 1% of apparently healthy persons, 6% of patients with nonmalignant disease, and 82% of patients with surgically demonstrated ovarian cancer had a CA125 level ≥ 35 U/ml [2, 25]. Here, MUC16 is linked to ovarian carcinoma.