Several human adrenal cell lines have previously been described.14 Of those, the H295R adrenocortical carcinoma cell line is the most established and used human cell line to model adrenal function.15 H295R cells are more akin to zonally undifferentiated human foetal adrenal cells that, unlike other cell lines described, retain their ability to produce adrenal steroid hormones and are responsive to agonists including angiotensin II and adrenocorticotropic hormone (ACTH).14 This makes H295R cells a useful in vitro tool for modelling steroidogenic pathways and processes.16–19. Here, POMC is linked to adrenal cortex carcinoma.