The results showed that in EVs extracted from hsa-miR-27a-3p mimic-treated GBM cells, upregulated CTGF prominently stimulated M2 macrophage polarization, in which the expression of hsa-miR-27a-3p (4.5-fold, p < 0.05, n = 3), KDM3A (2.2-fold, p < 0.05, n = 3), IL-10 (2.6-fold, p < 0.05, n = 3) and CTGF (2.2-fold, p < 0.05, n = 3) increased, but EZH1 (0.3-fold, p < 0.05, n = 3) and TNF-α (0.4-fold, p < 0.05, n = 3) expression reduced. Here, TNF is linked to glioblastoma.