In order to further determine that GBM-EVs-derived hsa-miR-27a-3p functioned as an upstream regulator of EZH1/KDM3A/CTGF in M2 macrophage polarization, CTGF was overexpressed in macrophages, followed by the determination of hsa-miR-27a-3p/EZH1/KDM3A/CTGF expression in each group. The gene discussed is EZH1; the disease is glioblastoma.