The requirement of ARL2 in the CSC of colon cancer was shown in two cancer cell lines, revealing that ARL2 knockdown‐induced genomic DNA break‐related stress and apoptosis are limited to the CSC population, while ARL2 knockdown arrests the cell cycle of BCC in the M phase, as previously suggested based on the known cytosolic effect of ARL2 on microtubules/centrosomes. Here, ARL2 is linked to cancer.