Furthermore, only patients with previous GVHD showed a significant increase in STAT3 (Ser727) phosphorylation in response to trans-signaling (hyper-IL-6), whereas only patients without GVHD showed highly significant (i.e., p < 0.01) increases in mTOR (Ser2448) phosphorylation in response to IL-6+sIL-6R (classical and trans-signaling) and IL-6+sIL-6R+sgp130FC (blockade of trans-signaling) (Table 3, Figure 4 left). This evidence concerns the gene STAT3 and graft versus host disease.