Decreases LDH activity and MDA, NSE and S100β contents; relaxes the cerebral basilar artery in a dose-dependent manner; up-regulates IP3, PKC, TRPV4, SKca and IKca expression; reduces Ca2+ fluorescence intensity; and ameliorates brain injury in rats with ischaemic stroke. The gene discussed is PRRT2; the disease is ischemic stroke.