ALB and lung carcinoma: In the presence of warfarin, the values of binding constants decreased, which suggest that derivative 3a could bind to HSA at the Sudlow site I. The findings presented in this paper suggest that these acridine derivatives exhibit promising potential as topoisomerase I and II inhibitors with anticancer activity against A549 human adherent lung carcinoma cells, and may also serve as DNA and HSA-interacting agents.