Several in vitro and in vivo studies have shown that N. sativa volatile oil exerts anti-cancer activity by targeting several signaling pathways, especially those involved in cell proliferation and apoptosis [21,49,50,51]; however, to our knowledge this is the first study that evaluates the inhibitory effect of N. sativa volatile oil on the epigenetic code of cancer cells regulated by the trimeric complex UHRF1/DNMT1/HDAC1. The gene discussed is HDAC1; the disease is cancer.