In conclusion, ethanol extract of Justicia spicigera exerted a protective effect on the livers of diabetic rats by reducing some characteristic symptoms of diabetes, such as hyperglycemia, body weight loss, serum triglycerides, and total serum lipids and by significantly reducing markers of hepatocyte injury such as gamma-glutamyl transpeptidase, alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase. Here, GPT is linked to diabetes mellitus.