Upstream regulators predicted to overlap with DEGs in HA-treated NEC pups included, among others, GUCY2C (guanylyl cyclase C), a regulator of intestinal barrier integrity [46], and TXNRD1 (thioredoxin reductase 1), an antioxidant scavenger associated with attenuation of intestinal barrier injury [47]. The gene discussed is TXNRD1; the disease is necrotizing enterocolitis.