LINC01234 and colorectal carcinoma: Using subcellular fractionation followed by real-time quantitative polymerase chain reaction (RT-qPCR) of CRC cell lines HCT116 and HCT15, we found that the RNA sequence LINC01234ORF (simplified as ORF) mainly localized to the cytoplasm (Figure 2D), and Western blot analysis showed the corresponding localization situation of MBOP (Figure 2E).