Here we focus on the effects of LDH-A depletion, both genetic (LDH-A shRNA) and pharmacologic (GNE-R-140), on i.c. tumor growth and animal survival, as well as the effects on nutrient depletion on tumor-cell metabolism and phenotype, by measuring changes in cellular bioenergetics by Seahorse Real-Time cell metabolic analysis. The gene discussed is LDHA; the disease is neoplasm.