These results show the combined impact of LDH-A depletion (LDH-A KD vs. NC control) and the immune system (C57BL/6 vs. nude mice) on the growth of s.c. located tumors and suggest that GL261 glioma cells (but not CT2A and ALTS1C1 cells) can adopt different metabolic pathways with higher TCA cycle activity under environmental stress, and that this capacity is enhanced by LDH-A depletion. This evidence concerns the gene LDHA and central nervous system cancer.