Six genetically altered murine glioma cell lines and tumors (with LDH-A shRNA knock-down (KD) vs. scrambled shRNA controls—NC) were compared for differences in their levels of LDH-A and LDH-B mRNA, protein and enzymatic activity, as well as their glycolytic and oxidative metabolic pathway activity. The gene discussed is LDHA; the disease is central nervous system cancer.