EGFR and non-small cell lung carcinoma: To study the effect of LPIN1 on gefitinib resistance in EGFR-activating mutant NSCLC cells, we determined the IC50 values of gefitinib in TKI-resistant H1650 cells stably transduced with LPIN1-specific or control shRNA and found that LPIN1-depleted H1650 cells exhibited an IC50 value approximately 5-fold lower than that of the control cells (Figure 1A).