CRISPR/Cas9 mediated gene knockout of NFKB1 in BJ fibroblast resulted in a significant reduction in stromal invasion vs. scrambled control, confirming that NFKB1 decreases stromal resistance to invasion (Figure 6B,C),and may contribute to the pro-invasable cluster 4 phenotype present in PDAC cancers with lymph metastasis. The gene discussed is NFKB1; the disease is cancer.