After 21 days of growth, gene expression of key markers associated with cancer-related processes were analysed by qPCR, including pluripotency (POU5F1, NANOG, SOX2 and NEAT1), epithelial-mesenchymal transition (EMT) (SNAI1, SNAI2, FOSL1, VIM and CDH2), proliferation (MKI67, CCNA2 and CCNB2), and breast cancer stem cell (CSC) (CD44, ALDHIA3 and ABCG2) related genes. The gene discussed is SNAI1; the disease is cancer.