NANOG and breast cancer: After 21 days of growth, gene expression of key markers associated with cancer-related processes were analysed by qPCR, including pluripotency (POU5F1, NANOG, SOX2 and NEAT1), epithelial-mesenchymal transition (EMT) (SNAI1, SNAI2, FOSL1, VIM and CDH2), proliferation (MKI67, CCNA2 and CCNB2), and breast cancer stem cell (CSC) (CD44, ALDHIA3 and ABCG2) related genes.