SLC38A1 and metastatic melanoma: To confirm the differential gene expression, we performed qRT-PCR with melanoma cell lines obtained from primary (Sbcl2, WM3211, WM1366, Mel Juso) and metastatic melanoma (WM1158, Mel Im, SKMel28), compared to NHEMs, demonstrating an upregulation of SNAT1/SLC38A1 gene expression, except for cell line Sbcl2 (Figure 1B).