-The level of TLR4, DUOX2, and NOX1 was upregulated in colon cancer cells-Gut microbiota activate TLR-4, which stimulates ROS production through Duox2 even after the inflammation is treated-Activation of TLR4 and DUOX2 increases the production of H2O2, which promotes tumor initiation. Here, TLR4 is linked to colonic neoplasm.