All 135 patients in this cohort were diagnosed with BRAF-mutant melanoma (13 patients tested positive for BRAF-V600K mutation, 105 tested positive for BRAF-V600E mutation, and in 17 patients PCR test was unable to distinguish between either BRAF-V600E/K mutation) and some patients showed an additional mutation, such as NRAS p.Q61 (n = 6) or EGFR (n = 1). This evidence concerns the gene NRAS and melanoma.