NPM1 and acute myeloid leukemia: For this, we selected F2, F3, and F4 of TSU and PSU sEVs derived from AML cell lines (OCI−AML3 cell line bearing NPM1 mutation and MV4−11 cell line bearing FLT3−ITD mutation) to perform mutational analysis as only these fractions revealed the presence of sEVs (Figure 2A).