Even if the combined measurement of circulating levels of cardiac-specific biomarkers usually shows better diagnostic and prognostic accuracy and also additive pathophysiological and clinical information in relation to classical invasive and non-invasive cardiac procedures in several clinical conditions (especially heart failure, myocardial injury and acute myocardial infarction), however, clinicians should be aware that both BNP/NT-proBNP and hs-cTn assays may show false (negative or positive) test results. The gene discussed is NPPB; the disease is heart failure.