Interestingly, the LGMD-R12 disease-causing gene ANO5 ranked in the top 0.1% of most downregulated genes, whereas other anoctamin genes showed a trend to being slightly upregulated (ANO3, ANO7, ANO9, ANO7L1) (Figure 3A). This evidence concerns the gene ANO7L1 and limb-girdle muscular dystrophy.