Interestingly, the LGMD-R12 disease-causing gene ANO5 ranked in the top 0.1% of most downregulated genes, whereas other anoctamin genes showed a trend to being slightly upregulated (ANO3, ANO7, ANO9, ANO7L1) (Figure 3A). The gene discussed is ANO9; the disease is limb-girdle muscular dystrophy.