Four breast (BT529, MCF7, MDA-MB-231 and MDA-MB-453), three lung (H460, Calu-1 and Calu-3), and two prostate cancer cells (DU145 and PC3) were investigated for the expression of pAkt1 and DNA-PKcs using western blotting. The gene discussed is PRKDC; the disease is Familial prostate cancer.