High-thoughput sequencing was performed in samples of patients with ACTH-independent and ACTH-dependent Cushing’s syndrome (due to cortisol-producing-adrenal adenoma; CPA or ACTH-secreting PitNET, ACTH-PA, respectively) and in controls where Cushing’s syndrome had been ruled out [81]. This evidence concerns the gene POMC and adrenal cortex adenoma.