Independent of previous studies on AKR1B10 and liver diseases [14,15,16,17,18,27], we found AKR1B10 via computational secretome analysis based on the DEGs according to NAFLD progression, and then showed that its gene and protein expression in the liver increased consistently and gradually according to disease progression via multiple approaches in our replication and validation cohorts. The gene discussed is AKR1B10; the disease is metabolic dysfunction-associated steatotic liver disease.