NOS1 and diabetes mellitus: In order to examine these changes, we assessed the immune-expression of nNOS by Western blot in 60 μg of total protein from pancreatic tissue fragments of normal and STZ-diabetic rats using anti-nNOS antibody at 12 h, 24 h, 1 week, 2 weeks, 1, 8 and 15 months after the onset of diabetes when compared to non-diabetic control.