BDNF and infection: We found significant upregulation of BDNF in the cerebral cortex of the Acanthamoeba spp.-infected immunocompetent mice (A) at 8 days post-infection (dpi), compared with the immunocompetent uninfected group (C) (U = 2; p = 0.03), while in the hippocampus, the BDNF level in the immunocompetent infected group (A) was higher at 8 dpi, lower at 16 dpi, and higher at 24 dpi (Figure 1) than the level in the uninfected group (C).