Western blot analysis of lysates from C8161, A375, and Sk-Mel28 treated for 72 h with 15 μM or 30 μM DAPT showed a significant increase in PS-1 expression compared with vehicle-treated melanoma cells used as control (fold increase: C8161, 15 μM = 2.0 +/− 0.18, 30 μM = 1.8 +/− 0.26; A375, 15 μM = 1.9 +/− 0.02, 30 μM = 1.7 +/− 0.07; Sk-Mel28, 15 μM = 1.6 +/− 0.01, 30 μM = 2.6 +/− 0.2) (p < 0.05) (Figure 3A). The gene discussed is PSEN1; the disease is melanoma.