In this work, we developed a new nanoparticle formulation containing non-carboxylated GRP (ucGRP), which comprises fluorescein-labeled CS/TPP nanoparticles produced by ionotropic gelation, and tested its anti-inflammatory properties in macrophage-differentiated THP1 (THP1-MoM), in human primary chondrocytes and VSMCs, as relevant cell systems involved in such CIDs as osteoarthritis and CVD. Here, GRP is linked to osteoarthritis.