As dozens of HOXA-AS2 isoforms are predicted by the Gencode gene project, we first assessed the expression of all HOXA-AS2 isoforms in three normal brain tissue samples (control), eight IDHwt and five IDHmut glioma samples, and two GSC lines using a strand-oriented RNA-seq approach. The gene discussed is HOXA-AS2; the disease is central nervous system cancer.