HOXA-AS2 and central nervous system cancer: RT-qPCR analysis (n = 10 control samples, n = 8 IDHmut and n = 43 IDHwt glioma samples, and n = 6 GSC lines) confirmed that HOXA-AS2 was expressed only in IDHwt glioma samples and GSC lines (Figure 1b), while it was virtually undetectable in control and IDHmut glioma samples.